Arthritis Research and Therapy publication: Ectopic bone formation and systemic bone loss in a transmembrane TNF-driven model of human spondyloarthritis

In this paper by Christodoulou-Vafeiadou et al., the MOLECUBES X-CUBE was used to investigate whether the TgA89 mouse model reproduces key pathological features of human spondylitis including axial and peripheral ectopic bone formation and systemic bone loss.

Arthritis Research & Therapy. 2020; 22:232. doi: 10.1186/s13075-020-02327-4.

Abstract

Research question

Spondyloarthritis described a family of of inflammatory rheumatic diseases that cause arthritis and is characterised by inflammation of peripheral joints and spine as well as bone destruction and deformation. The transmembrane-TNF transgenic mouse, TgA86, has been shown to develop spontaneously peripheral arthritis with signs of axial involvement. To assess similarity to human spondyloarthritis, we performed detailed characterization of the axial, peripheral, and comorbid pathologies of this model.

Experiment

TgA86 bone pathologies were assessed at different ages using CT imaging of the spine, tail vertebrae, and hind limbs and characterized in detail by histopathological and immunohistochemical analysis. Cardiac function was examined by echocardiography and electrocardiography and bone structural parameters by μCT analysis. The response of TgA86 mice to either early or late anti-TNF treatment was evaluated clinically, histopathologically, and by μCT analysis.

Results

TgA86 mice developed with 100% penetrance spontaneous axial and peripheral pathology which progressed with time and manifested as reduced body weight and body length, kyphosis, tail bendings, as well as swollen and distorted hind joints. Whole-body CT analysis at advanced ages revealed bone erosions of sacral and caudal vertebrae as well as of sacroiliac joints and hind limbs and, also, new ectopic bone formation and eventually vertebral fusion. The pathology of these mice highly resembled that of SpA patients, as it evolved through an early inflammatory phase, evident as enthesitis and synovitis in the affected joints, characterized by mesenchymal cell accumulation, and neutrophilic infiltration. Subsequently, regression of inflammation was accompanied by ectopic bone formation, leading to ankylosis. In addition, both systemic bone loss and comorbid heart valve pathology were evident. Importantly, early anti-TNF treatment, similar to clinical treatment protocols, significantly reduced the inflammatory phase of both the axial and peripheral pathology of TgA86 mice.

 

 

 

 

 

 

 

 

The TgA86 mice thus develop a spontaneous peripheral and axial biphasic pathology accompanied by comorbid heart valvular dysfunction and osteoporosis, overall reproducing the progression of pathognomonic features of human spondyloarthritis. Therefore, the TgA86 mouse represents a valuable model for deciphering the role of transmembrane TNF in the pathogenic mechanisms of spondyloarthritis and for assessing the efficacy of human therapeutics targeting different phases of the disease.

 

KEYWORDS: Spondyloarthritis, Ectopic bone formation, TNF, Inflammation, Animal disease model, computed tomography, CT imaging, small animal imaging