Quantitative cardiac gated PET imaging

In cardiac research, non-invasive measurement of left ventricular end-systolic (ESV), end-diastolic volume (EDV) and left ventricular ejection fraction (LVEF) is a useful tool to evaluate and monitor cardiac function in health and disease.

In order to obtain physiological signals, Molecubes developed dedicated animal beds for mice and rats with fully integrated cardiac, respiratory and temperature monitoring capabilities. The electronics are equipped with a high precision clock to allow synchronisation of the monitoring signals with the CUBES. In the example below, a rat was injected with 17 MBq of [18F]-FDG 80 min prior to cardiac gated PET scanning (dose at start scan: 10.2 MBq). A PET acquisition of 45 minutes was done. 3 ECG-leads connected to the rat front legs and left hind leg were used to obtain the cardiac signal. For each cardiac cycle, 8 gates were defined and for each gate a PET image was reconstructed using the OSEM algorithm with default settings as provided by the Molecubes software. ESV, EDV and LVEF were calculated using PMOD PCARD v4.0. For cardiac gated PET, a left ventricular ejection fraction of 68.54% was calculated based on EDV and ESV in a healthy rat. Thanks to the high spatial resolution of the PET system, cardiac function can be visualised and quantified.